FITC标记的鸟苷酸环化酶α2/GCS-α-2抗体
产品名称： FITC标记的鸟苷酸环化酶α2/GCS-α-2抗体
英文名称： Anti-Guanylyl Cyclase alpha 2/FITC
产品编号： HZ-
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-Guanylyl Cyclase alpha 2/FITC Conjugated antibody
FITC标记的鸟苷酸环化酶α2/GCS-α-2抗体
| 英文名称 | Anti-Guanylyl Cyclase alpha 2/FITC |
| 中文名称 | FITC标记的鸟苷酸环化酶α2/GCS-α-2抗体 |
| 别 名 | GC SA2; GCS-alpha 2; GUC1A2; GUCSA2; GCYA2_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 神经生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 83kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Guanylyl Cyclase alpha 2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase. The soluble form, known as GCS or sGC, act as receptors for nitric oxide (NO). The membrane-bound receptor form, known as GC, are peptide hormone receptors. GCS is a cGMP-synthesizing enzyme, which is the major receptor for the neurotransmitter nitric oxide. It plays a crucial role in smooth muscle contractility, platelet reactivity and neurotransmission. GCS is a heme-containing heterodimer, consisting of one alpha subunit and one beta subunit. The heme moeity mediates NO activation, and this heme group also binds carbon monoxide (CO), which weakly stimulates the enzyme. Both NO and CO stimulation are enhanced by the allosteric activator 3-(5'-hydroxymethyl-2'furyl)-benzyl-indazole, YC-1. YC-1 can also stimulate GCS in a NO-independent manner. Both alpha and beta subunits are required for cGMP generation, and at least two isoforms exist for each subunit. Heterodimers consisting of alpha-1/beta-1 and alpha-2/beta-1 have been identified, and both display similar enzymatic activity. The distribution of the beta-2 subunit seems to be much more restricted than the beta-1 subunit, with predominant expression in kidney and liver. Function: The cellular levels of Cyclic GMP (cGMP), a key messenger in several signal transduction pathways, are maintained by the orchestrated activities of two opposing enzymes: synthesizing gualylyl cyclases (GC) and hydrolyzing phosphodiesterases (PDEs). Guanylyl cyclases exist in membrane-bound and cytoplasmic forms. The cDNAs of two alpha and two beta subunits have been identified. The known membrane-spanning forms are cell surface receptors for extracellular peptides and appear to exist as homodimers or other higher ordered structures. The cytoplasmic or soluble forms represent heme-containing heterodimers that are primary receptors for nitric oxide (NO) generated by NO synthases are obligate heterodimers consisting of an alpha and a beta subunit. Guanylate cyclase soluble subunit alpha 2 has guanylyl cyclase on binding to the beta1 subunit. The alternatively spliced isoform alpha2I acts as a negative regulator of guanylyl cyclase activity as it forms non-functional heterodimers with the beta subunits. Subunit: Heterodimer of an alpha and a beta chain. Subcellular Location: Cytoplasm. Tissue Specificity: Isoform 1 is expressed in fetal brain, liver, colon, endothelium and testis. Isoform 2 is expressed only in liver, colon and endothelium. Similarity: Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 1 guanylate cyclase domain. Database links: UniProtKB/Swiss-Prot: P33402.1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
鸟苷酸环化酶属于腺苷酸环化酶类4/鸟苷酸环化酶家族。鸟苷酸环化酶有两种形式。可溶形式，称为GCS或SGC，作为一氧化氮（NO）的受体。膜结合的受体形式，称为GC，是肽类受体。GCS是一种cGMP合成酶，它是神经递质一氧化氮的主要受体。它在平滑肌收缩性、血小板反应性和神经传递中起着至关重要的作用。GCS是一种含血红素的异源二聚体，由一个α亚基和一个β亚基组成。血红素不介导激活，并且血红素组也结合一氧化碳（CO），这弱刺激酶。NO和CO刺激均由变构激活剂3（5’-羟甲基-2’呋喃基）-苄基吲唑，YC-1增强。YC-1也可以以不独立的方式刺激GCS。α和β亚基都是cGMP生成所必需的，并且每个亚基至少存在两种亚型。已鉴定出由α-1／β1和α-2 /BETA-1组成的异源二聚体，它们均显示类似的酶活性。β-2亚基的分布似乎比β-1亚基受限制得多，在肾脏和肝脏中主要表达。