FITC标记的肌动蛋白解聚因子抗体
产品名称： FITC标记的肌动蛋白解聚因子抗体
英文名称： Anti-Destrin/FITC
产品编号： HZ-12997R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-Destrin/FITC Conjugated antibody
FITC标记的肌动蛋白解聚因子抗体
| 英文名称 | Anti-Destrin/FITC |
| 中文名称 | FITC标记的肌动蛋白解聚因子抗体 |
| 别 名 | 2610043P17Rik; ACTDP; Actin depolymerizing factor; Actin-depolymerizing factor; ADF; AU042046; bA462D18.2; corn1; DEST_HUMAN; Destrin (actin depolymerizing factor); Destrin; DSN; Dstn; Sid 23; sid23p. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 信号转导 结合蛋白 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Monkey, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 19kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Destrin |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Actin-depolymerizing factor (ADF), also known as destrin, is a member of the ADF/Cofilin/destrin superfamily that has the ability to rapidly depolymerize F-Actin in a stoichiometric manner. The Actin-depolymerizing activity of ADF is reversibly controlled by changes in KCl concentration but is insensitive to calcium concentration. ADF depolymerizes F-Actin by interacting directly with F-Actin protomers. ADF shares 71% sequence homology with Cofilin, however the two proteins differ in their interaction with Actin. The difference in the function of ADF and Cofilin results from the subtle difference in their amino acid sequence rather than possible differences in posttranslational modifications. As a result of different cleavage sites on ADF and Cofilin, the proteins differ in their overall tertiary folds. Sensitivity to polyphosphoinositides may be a common feature in vitro among Actin-binding proteins such as ADF and Cofilin that can bind to G-Actin and regulate the state of Actin polymerization. ADF and Cofilin are Actin-depolymerizing proteins whose activities are possibly regulated by their phosphorylation/dephosphorylation. Function: Actin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Acts in a pH-independent manner. Tissue Specificity: Widely distributed in various tissues. Post-translational modifications: ISGylated. Similarity: Belongs to the actin-binding proteins ADF family. Contains 1 ADF-H domain. Database links: UniProtKB/Swiss-Prot: P60981.3 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
肌动蛋白解聚因子(ADF)，又称destrin，是ADF/Cofilin/destrin超家族的成员，具有以化学计量方式快速解聚F-肌动蛋白的能力。ADF的肌动蛋白解聚活性受KCl浓度变化的可逆控制，但对钙浓度不敏感。ADF通过直接与F-肌动蛋白原质子相互作用来解聚F-肌动蛋白。ADF与COFILIN共有71%个序列同源性，但这两个蛋白在肌动蛋白的相互作用上不同。ADF和Cofilin功能的差异是由于它们的氨基酸序列的细微差异而不是翻译后修饰的可能差异。由于ADF和CoFILIN上的不同切割位点，蛋白质在它们的整体第三折叠中不同。对多磷酸肌苷的敏感性可能是肌动蛋白结合蛋白（如ADF和Cofilin）的一个共同特征，这些蛋白可以结合G-肌动蛋白并调节肌动蛋白聚合的状态。ADF和Cofilin是肌动蛋白解聚蛋白，其活性可能受其磷酸化/去磷酸化的调节。